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Publications

Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay. Siyi Lai, Shengnian Wang, Jun Luo, L. James Lee, Shang-Tian Yang, and Marc J. Madou. Abstract: This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method. Analytical Chemistry, 76(7), 1832-1837, 2004.

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Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions. Marc J. Madou, L. James Lee, et. al. Abstract: In this paper, the design of a polymer based microfluidic compact disk (CD) platform is presented. Several microfluidic functions such as flow sequencing, cascade micro-mixing, and capillary metering can be integrated into the CD by balancing the centrifugal force and the capillary force. These functions are demonstrated experimentally. For flow sequencing, a two-point calibration design is used as an example to show how the release and flow of fluids can be precisely controlled by the rotation speed of the CD. For cascade micro-mixing, a typical application is reconstituting lyophilized protein. A simple metering technique based on bubble snap-off in the two-phase flow is also described. Biomedical Microdevices 3:3, 245-254, 2001.

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A Centrifugal Microfluidic platform- A comparison. Madou MJ, Lu Y, Lai S, Lee LJ, Daunert S.. In: van den Berg A, Olthuis W, Bergveld P, editors. Micro Total Analysis Systems Boston: Academic Publishers, 2000: 565-570.